Microbiome Diversity Reporter

Interpret Alpha and Beta diversity metrics from 16S rRNA sequencing results.

---

When to Use

• Use this skill when the task needs Interpret Alpha and Beta diversity metrics from 16S rRNA sequencing results.
• Use this skill for academic writing tasks that require explicit assumptions, bounded scope, and a reproducible output format.
• Use this skill when you need a documented fallback path for missing inputs, execution errors, or partial evidence.

Key Features

• Scope-focused workflow aligned to: Interpret Alpha and Beta diversity metrics from 16S rRNA sequencing results.
• Packaged executable path(s): scripts/main.py.
• Reference material available in references/ for task-specific guidance.
• Structured execution path designed to keep outputs consistent and reviewable.

Dependencies

• Python 3.8+
• numpy
• pandas
• scipy
• scikit-bio
• matplotlib
• seaborn
• plotly (for interactive charts)

---

Example Usage

See ## Usage above for related details.

bash

cd "20260318/scientific-skills/Academic Writing/microbiome-diversity-reporter"
python -m py_compile scripts/main.py
python scripts/main.py --help

Example run plan:

1. Confirm the user input, output path, and any required config values.
2. Edit the in-file CONFIG block or documented parameters if the script uses fixed settings.
3. Run python scripts/main.py with the validated inputs.
4. Review the generated output and return the final artifact with any assumptions called out.

Implementation Details

See ## Workflow above for related details.

• Execution model: validate the request, choose the packaged workflow, and produce a bounded deliverable.
• Input controls: confirm the source files, scope limits, output format, and acceptance criteria before running any script.
• Primary implementation surface: scripts/main.py.
• Reference guidance: references/ contains supporting rules, prompts, or checklists.
• Parameters to clarify first: input path, output path, scope filters, thresholds, and any domain-specific constraints.
• Output discipline: keep results reproducible, identify assumptions explicitly, and avoid undocumented side effects.

Quick Check

Use this command to verify that the packaged script entry point can be parsed before deeper execution.

bash

python -m py_compile scripts/main.py

Audit-Ready Commands

Use these concrete commands for validation. They are intentionally self-contained and avoid placeholder paths.

bash

python -m py_compile scripts/main.py
python scripts/main.py --help
python scripts/main.py -h

Workflow

1. Confirm the user objective, required inputs, and non-negotiable constraints before doing detailed work.
2. Validate that the request matches the documented scope and stop early if the task would require unsupported assumptions.
3. Use the packaged script path or the documented reasoning path with only the inputs that are actually available.
4. Return a structured result that separates assumptions, deliverables, risks, and unresolved items.
5. If execution fails or inputs are incomplete, switch to the fallback path and state exactly what blocked full completion.

Overview

This tool is used to analyze and interpret diversity metrics in microbiome 16S rRNA sequencing data, including:

• Alpha Diversity: Species diversity within a single sample
• Beta Diversity: Species composition differences between samples

---

Usage

Command Line

text

# Analyze Alpha diversity for a single sample
python scripts/main.py --input otu_table.tsv --metric shannon --output alpha_report.html

# Analyze Beta diversity (PCoA)
python scripts/main.py --input otu_table.tsv --beta --metadata metadata.tsv --output beta_report.html

# Generate full report (Alpha + Beta)
python scripts/main.py --input otu_table.tsv --full --metadata metadata.tsv --output diversity_report.html

Parameter Description

Parameter	Description	Required
--input	OTU/ASV table path (TSV format)	Yes
--metadata	Sample metadata (TSV format)	Required for Beta diversity
--metric	Alpha diversity metric: shannon, simpson, chao1, observed_otus	No (default: shannon)
--alpha	Calculate Alpha diversity only	No
--beta	Calculate Beta diversity only	No
--full	Generate full report (Alpha + Beta)	No
--output	Output report path	No (default: stdout)
--format	Output format: html, json, markdown	No (default: html)

---

Input Format

OTU Table (TSV)

text

#OTU ID	Sample1	Sample2	Sample3
OTU_1	100	50	200
OTU_2	50	100	0
OTU_3	25	25	50

Metadata (TSV)

text

SampleID	Group	Age	Gender
Sample1	Control	25	M
Sample2	Treatment	30	F
Sample3	Treatment	28	M

---

Output

Generates HTML/JSON/Markdown reports containing:

1. Alpha Diversity Results

   • Diversity index values
   • Rarefaction curves
   • Box plots (by group)

2. Beta Diversity Results

   • PCoA scatter plots
   • NMDS plots
   • Distance matrix heatmaps
   • PERMANOVA statistical tests

3. Statistical Summary

   • Sample information statistics
   • Species richness
   • Diversity index distribution

---

Example Output

json

{
  "alpha_diversity": {
    "shannon": {
      "Sample1": 2.45,
      "Sample2": 1.89,
      "Sample3": 2.12
    },
    "statistics": {
      "mean": 2.15,
      "std": 0.28
    }
  },
  "beta_diversity": {
    "method": "braycurtis",
    "pcoa": {
      "variance_explained": [0.45, 0.25, 0.15]
    }
  }
}

---

References

1. Shannon, C.E. (1948) A mathematical theory of communication
2. Simpson, E.H. (1949) Measurement of diversity
3. Chao, A. (1984) Non-parametric estimation of classes
4. Lozupone et al. (2005) UniFrac: a phylogenetic metric

Risk Assessment

Risk Indicator	Assessment	Level
Code Execution	Python/R scripts executed locally	Medium
Network Access	No external API calls	Low
File System Access	Read input files, write output files	Medium
Instruction Tampering	Standard prompt guidelines	Low
Data Exposure	Output files saved to workspace	Low

Security Checklist

• No hardcoded credentials or API keys
• No unauthorized file system access (../)
• Output does not expose sensitive information
• Prompt injection protections in place
• Input file paths validated (no ../ traversal)
• Output directory restricted to workspace
• Script execution in sandboxed environment
• Error messages sanitized (no stack traces exposed)
• Dependencies audited

Prerequisites

text

# Python dependencies
pip install -r requirements.txt

Evaluation Criteria

Success Metrics

• Successfully executes main functionality
• Output meets quality standards
• Handles edge cases gracefully
• Performance is acceptable

Test Cases

1. Basic Functionality: Standard input → Expected output
2. Edge Case: Invalid input → Graceful error handling
3. Performance: Large dataset → Acceptable processing time

Lifecycle Status

• Current Stage: Draft
• Next Review Date: 2026-03-06
• Known Issues: None
• Planned Improvements:
  • Performance optimization
  • Additional feature support

Output Requirements

Every final response should make these items explicit when they are relevant:

• Objective or requested deliverable
• Inputs used and assumptions introduced
• Workflow or decision path
• Core result, recommendation, or artifact
• Constraints, risks, caveats, or validation needs
• Unresolved items and next-step checks

Error Handling

• If required inputs are missing, state exactly which fields are missing and request only the minimum additional information.
• If the task goes outside the documented scope, stop instead of guessing or silently widening the assignment.
• If scripts/main.py fails, report the failure point, summarize what still can be completed safely, and provide a manual fallback.
• Do not fabricate files, citations, data, search results, or execution outcomes.

Input Validation

This skill accepts requests that match the documented purpose of microbiome-diversity-reporter and include enough context to complete the workflow safely.

Do not continue the workflow when the request is out of scope, missing a critical input, or would require unsupported assumptions. Instead respond:

microbiome-diversity-reporter only handles its documented workflow. Please provide the missing required inputs or switch to a more suitable skill.

References

• references/audit-reference.md - Supported scope, audit commands, and fallback boundaries

Response Template

Use the following fixed structure for non-trivial requests:

1. Objective
2. Inputs Received
3. Assumptions
4. Workflow
5. Deliverable
6. Risks and Limits
7. Next Checks

If the request is simple, you may compress the structure, but still keep assumptions and limits explicit when they affect correctness.